If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. All claims must be made within 30 days following the date of delivery. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All properties listed are typical characteristics and are not specifications. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The unbound antibody is washed and the sample containing target antigen is added. For IP, target specific antibody is incubated with rabbit Anti-goat IgG magnetic beads. The beads are then magnetically separated from the eluted solution, which is removed manually. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. For antibody purification, rabbit Anti-goat IgG magnetic beads are incubated with the goat antibody solution and then separated by magnets. Anti-Goat IgG Magnetic Beads has been tested in SDS-Page, immunoprecipitation, and western blot. TrueBlot® rabbit Anti-goat IgG magnetic beads can be used for separation and purification of goat antibodies from serum or goat antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. The beads are in suspension and will settle upon storage. The beads have a large surface area with high capture efficiencies. Cell separation and sorting can be achieved using a goat IgG antibody to defined cell surface antigens. This antibody binds the heavy chain of goat IgG and is suitable for immunoassays that utilize a goat IgG primary polyclonal antibody. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as rabbit Anti-goat IgG, and are specifically designed, tested and quality controlled for isolation and purification of goat IgG, and immunoprecipitation methods using manual or automatic platforms. TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups.
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